บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Prolongation of vase life of cut roses via introduction of genes coding for antibacterial activity. Somatic embryogenesis and Agrobacterium-mediated transformation.

Derks, F. H. M.; Dijk, A. J. van; Hanisch ten Cate, C. H.; Florack, D. E. A.; Dubois, L. A. M.; Vries, D. P. de;

Acta Horticulturae Year: 1995 Issue: No. 405 Pages: 205-209 Ref: 14 ref.

1995

บทคัดย่อ

Prolongation of vase life of cut roses via introduction of genes coding for antibacterial activity. Somatic embryogenesis and Agrobacterium-mediated transformation.

ABSTRACT :

 

A reproducible regeneration method via somatic embryogenesis was developed for various rose (Rosa hybrida) cultivars. In vitro induced roots were cultured on medium with high concentrations of auxins followed by culture on hormone-less medium. Embryogenic callus with somatic embryos was obtained, which regenerated into normal plantlets. Transformation of embryogenic callus of 3 cultivars was performed with Agrobacterium tumefaciens harbouring a binary vector containing a kanamycin resistance gene, a GUS gene with an intron and a gene encoding the antibacterial cecropin B. Transgenic callus was obtained for all binary vectors used. Transgenic somatic embryos were induced on transgenic callus of cv. Sonia.