Mode of action of Bacillus subtilis for control of avocado post-harvest pathogens.
Korsten, L.; Jager, E. E. de;
Yearbook - South African Avocado Growers' Association Year: 1995 Vol: 18 Pages: 124-130 Ref: 24 ref.
1995
บทคัดย่อ
ABSTRACT :
B. subtilis (isolate B246), B. cereus (isolates B247 and B249) and B. licheniformis (isolate B248) were inhibitory to the avocado postharvest pathogens Colletotrichum gloeosporioides [Glomerella cingulata], Phomopsis perseae, Drechslera setariae [Cochliobolus setariae], Pestalotiopsis versicolor and Fusarium solani when tested using the dual culture technique. In vitro growth of Thyronectria pseudotrichia was not suppressed by any of the antagonists, while B. cereus isolate B247 did not inhibit growth of Dothiorella aromatica. B. cereus isolate B249 stimulated development of Lasiodiplodia [Botryodiplodia] theobromae. Diffusible metabolites from the 4 antagonists impeded the growth of G. cingulata, D. aromatica, P. perseae and B. theobromae, but not that of T. pseudotrichia. The mode of action of B. subtilis (B246) was determined using an in vitro spore-germination assay. In vitro the antagonist effectively reduced the % spore germination of G. cingulata, D. aromatica, P. perseae, B. theobromae,
F. solani, P. versicolor and D. setariae. In addition, bulb formation and lysis of hyphae were observed, as well as bacterial movement towards germinating spores and subsequent attachment to the spores. The possible mode of action of B. subtilis is discussed.