บทคัดย่องานวิจัย

Membrane lipids involved in the regulation of flower senescence.

Borochov, A.; Spiegelstein, H.; Porat, R.;

Acta Horticulturae Year: 1995 Issue: No. 405 Pages: 240-245 Ref: 11 ref.

1995

บทคัดย่อ

Membrane lipids involved in the regulation of flower senescence.

 

Flower senescence is accompanied by a decline in the content of membrane components, including nonspecific decreases in phospholipids. The possible involvement of products of phospholipid metabolism in the regulation of petunia flower senescence was investigated using cv. Pink Cascade. During cut flower development and senescence, a transient increase in the content of diacylglycerol (DAG) in the petal membranes was found, peaking towards wilting. Phorbol 12-myristate 13-acetate (PMA), a phorbol ester, is water-soluble and acts in various cellular metabolic processes, including kinase activation. PMA is an analogue of DAG, and therefore serves as a useful tool for studying DAG's role in cell metabolism. Treating petunia flowers with 5-20 micro M PMA enhanced wilting in a concentration-dependent manner. In addition, flower FW and protein content declined earlier in PMA-treated flowers. The membrane lipid microviscosity of PMA-treated flowers increased faster than that of control flowers and the t

reatment induced earlier ethylene production. Pre-treatment with STS nullified the effects of PMA on flower senescence. These results indicate a role for lipid metabolites in the regulation of flower senescence and support the possible role of protein kinase activity in this process.