ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Plant defensive proteins and disease resistance in citrus.

Niedz, R. P.; Doostdar, H.; McCollum, T. G.; McDonald, R. E.; Mayer, R. T.;

Proceedings of the Florida State Horticultural Society Year: 1994, recd. 1995 Issue: No. 107 Pages: 79-82 Ref: 12 ref.

1995

บทคัดย่อ

Tissue cultures of sweet orange embryogenic callus and suspension cells can be induced to produce high levels of chitinase and beta -1,3-glucanase by altering the carbon source in the culture medium. To determine if these enzymes inhibit disease-causing fungi, Penicillium digitatum, a postharvest pathogen, was inoculated onto Petri dishes with sweet orange callus that had been overgrown on media containing either sucrose or glycerol as the carbon source. Medium that contained sucrose induced high levels of chitinase and beta -1,3-glucanase in the callus, which remained undifferentiated. Medium that contained glycerol repressed the production of these enzymes in the callus, which differentiated into embryos. Fungal growth was significantly inhibited by the callus producing high levels of enzyme for up to 6 weeks. Chitinase and beta -1,3-glucanase have been identified in the roots, leaves and flavedo of Marsh grapefruit, and in the leaves and flowers of navel orange. These enzymes might be useful

in developing new economical and environmentally friendly disease control measures.