Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves.
Meir, S.; Kanner, J.; Akiri, B.; Philosoph-Hadas, S.;
Journal of Agricultural and Food Chemistry Year: 1995 Vol: 43 Issue: 7 Pages: 1813-1819 Ref: 27 ref.
1995
บทคัดย่อ
A method for determining different types of reducing activity in aqueous leaf extracts of various edible herb species (watercress, parsley (Petroselinum crispum), basil (Ocimum basilicum) and sage (Salvia officinalis)) was developed. This method, based on kinetics of the reduction of Fe(III) to Fe(II), was employed to study the correlation between leaf senescence rates under darkness and their relative reducing capacity in aqueous extracts, which represents an oxidative defence mechanism. Evidence is presented showing that total reducing capacity is negatively correlated with relative storage potential. Also, the initiation of senescence processes was associated with a weakening of the oxidative defence system. Exogenous application of active reducing agents (morin, ferulic acid, kaempferol and glutathione) significantly inhibited chlorophyll degradation of detached parsley leaves to an extent related to their in vitro effectiveness of reducing activity. The results emphasize the correlation bet
ween decreasing senescence rate and increasing reducing activity, thereby confirming the possible involvement of reducing compounds in oxidative defence systems of various edible senescing leaves.