บทคัดย่องานวิจัย

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Immunodetection of Botrytis allii, the causal agent of neck rot of onion bulbs.

Linfield, C. A.; Lyons, N. F.; Kenny, S. R.;

Bulletin OEPP Year: 1995 Vol: 25 Issue: 1/2 Pages: 39-46 Ref: 11 ref.

1995

บทคัดย่อ

Immunodetection of Botrytis allii, the causal agent of neck rot of onion bulbs.

 

Polyclonal antisera were raised from cell wall, cytoplasmic and surface plate washings of 2 B. allii isolates and used to develop an indirect ELISA for detection of latent infection in stored onion bulbs. The antisera did not react with healthy onion tissue and showed only min. recognition of fungi from other genera. Some cross reactivity was observed with B. cinerea but not with other Botrytis spp. tested. In many instances following infection, fungal antigen could be detected earlier by ELISA than by direct plating. In tests on bulbs from a commercial store, the level of B. allii infection was 3.3% with ELISA and direct plating, but the result was achieved 7 d earlier with ELISA.