บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Competitive ELISA of thiabendazole residues in produce using indirectly immobilized monoclonal antibodies.

Brandon, D. L.; Binder, R. G.; Bates, A. H.; Montague, W. C., Jr.;

Food and Agricultural Immunology Year: 1995 Vol: 7 Issue: 2 Pages: 99-108 Ref: 18 ref.

1995

บทคัดย่อ

Competitive ELISA of thiabendazole residues in produce using indirectly immobilized monoclonal antibodies.

 

An ELISA originally developed for analysis of thiabendazole residues in bovine liver, was used to analyse residues of this postharvest fungicide in the peel of apple, potato, orange, grapefruit and banana. Residues were extracted by soaking peels overnight in 80% methanol and filtering the decanted supernatants. A 20-fold dilution eliminated significant matrix effects. The ELISA has a limit of detection of 0.1 p.p.m. in peel samples, corresponding to 10-40 p.p.b. in the whole fruit or tuber. The ELISA uses a monoclonal antibody layer, immobilized indirectly via a rabbit anti-mouse IgG secondary antibody and horseradish peroxidase-conjugated 5-succinamido-thiabendazole. It is suggested that assay wells coated in this way could be dried and stored for at least 6 months without detectable changes in assay parameters. It is concluded that the method exceeds the required sensitivity for regulatory monitoring of thiabendazole residues in the USA and offers greater speed and specificity than previously

 reported methods.