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Cryo SEM study of changes in tissue anatomy of harvested sporophores of Agaricus bisporus.

Evered, C. E.; Burton, K. S.;

Mushroom science XIV, Volume 2. Proceedings of the 14th international congress on the science and cultivation of edible fungi, Oxford, UK, 17-22 Sep. 1995. Year: 1995 Pages: 717-721 Ref: 11 ref.

1995

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Cryo SEM study of changes in tissue anatomy of harvested sporophores of Agaricus bisporus.

 

Cryo-fracture was used to observe mushrooms under a scanning electron microscope. The technique revealed anatomical details of sporophore tissues without the problem of exudate smearing and cell displacement. During postharvest senescence at 18.5 deg C, a proportion of cells underwent total collapse while the remaining cells remained fully turgid. The number of cells autolysing in this way increased during the storage period leaving a network of intact cells and greatly enlarged intercellular spaces. Mushroom senescence appeared to be localized and controlled at the level of individual cells.