Biocontrol of postharvest diseases of apple using Bacillus spp. isolated from stored apples.
Sholberg, P. L.; Marchi, A.; Bechard, J.;
Canadian Journal of Microbiology Year: 1995 Vol: 41 Issue: 3 Pages: 247-252 Ref: 23 ref.
1995
บทคัดย่อ
Ninety-five bacterial isolates were recovered from 38 of 77 apples that had been stored at 1 deg C for 6-7 months. The highest number of bacteria were recovered in nutrient, dextrose, and V8 juice broths, resp. The bacteria were screened as biocontrol agents on cultivar Red Delicious apples primarily for control of blue mould caused by Penicillium expansum. Three bacteria effective against P. expansum were also tested against Botrytis cinerea for control of gray mould. Ten, four, and five isolates significantly reduced blue mould decay when apples were stored at 5, 10, and 20 deg C. Two isolates tested against gray mould decay significantly reduced decay at 5 and 10 deg C and one isolate was effective at 20 deg C. Thirty-six isolates that had been selected for identification by the Biolog MicrostationTM System were gram positive and contained endospores, and 30 of these were positively identified as Bacillus spp. Further testing of 15 isolates that were effective biocontrol agents identified 7 a
s Bacillus subtilis on the basis of 15 microbiological tests used for determining species within the genus Bacillus.