บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

beta -D-Galactosidase and alpha -L-arabinofuranosidase activities during the softening of apples.

Yoshioka, H.; Kashimura, Y.; Kaneko, K.;

Journal of the Japanese Society for Horticultural Science Year: 1995 Vol: 63 Issue: 4 Pages: 871-878 Ref: 27 ref.

1995

บทคัดย่อ

beta -D-Galactosidase and alpha -L-arabinofuranosidase activities during the softening of apples.

 

Activity of 4 beta -D-galactosidase (GA-ase I, II, III and IV) and one alpha -L-arabinofuranosidase (AF-ase) was detected in cell wall extracts from apple cv. Starking Delicious fruits stored at 0 deg C for 5 months. The GA-ase fractions separated by hydroxylapatite column chromatography degraded a polyuronide prepared from fruit cell walls, releasing galactose. GA-ase II, III and IV fractions contained arabinogalactan-degrading and galactose-releasing activities, but GA-ase I failed to degrade arabinogalactan. AF-ase fraction degraded polyuronide and arabinogalactan, releasing arabinose and other sugars. The activities of GA-ase II, III and IV decreased gradually as the fruits softened in storage. The activities of GA-ase I and AF-ase, which were not detectable at harvest, appeared and increased during storage. It was concluded that these galactose and arabinose releasing activities may be involved in the degradation and solubilization of polyuronide, araban and galactan in the cell walls of ap

ples during softening.