ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Involvement of ethylene in fruit ripening: expression and control of ACC oxidase gene.

Pech, J. C.; Lasserre, E.; Ayub, R.; Guis, M.; Bidonde, S.; Hernandez, J. A.; Ramassamy, S.; Rombaldi, C. V.; Bouzayen, M.; Balague, C.; Latche, A.;

Proceedings of the Australasian postharvest horticulture conference 'Science and technology for the fresh food revolution', Melbourne, Australia, 18-22 September 1995. Year: 1996 Pages: 47-53 Ref: 20 ref.

1996

บทคัดย่อ

The ripening of climacteric fruits is associated with a sharp increase in ethylene production and in the activities of both ACC synthase and ACC oxidase. This paper reports research specifically devoted to ACC oxidase aimed at (i) elucidating the subcellular localization of the protein, (ii) characterizing the corresponding gene family, and (iii) manipulating ethylene synthesis and fruit ripening with the antisense ACC oxidase gene. New evidence is provided for the location of ACC oxidase in the cell wall in ripening fruits using protoplasts and fractionation methods. It is present as three isoforms corresponding to the expression of a small gene family. Three genomic clones (CM-ACO1, CM-ACO2 and CM-ACO3) encoding ACC oxidase were isolated and characterized from melon. Transient expression studies showed that the CM-ACO1 promoter is capable of stimulating expression of the GUS reporter gene upon ethylene treatment, demonstrating that it contains ethylene-responsive sequences that could be involv

ed in the autocatalytic stimulation of ethylene production observed in ripening fruit. In order to slow-down ripening, melon plants were transformed via Agrobacterium tumefaciens with antisense ACC oxidase cDNA, and transgenic fruit showing more than 95% reduction of ethylene production were obtained. The physiological behaviour of these fruits, both on and off the vine, is described.