Ultrastructural cytology of superficial scald in Granny Smith apples.
Golding, J.; Sarafis, V.; McGlasson, B.; Nailon, J.; Cribb, B.;
Proceedings of the Australasian postharvest horticulture conference 'Science and technology for the fresh food revolution', Melbourne, Australia, 18-22 September, 1995. Year: 1996 Pages: 17-21 Ref: 14 ref.
1996
บทคัดย่อ
Ultrastructural changes associated with scald in fruits stored for 6 months were examined using transmission electron microscopy following conventional chemical fixation or cryofixation. Examination of tissue fixed by these methods confirmed previously published TEM observations. Scald symptoms were associated with an electron-dense deposit on the tonoplast of scalded cells. However, contrary to expectations, the chloroplast membranes in scalded cells appeared to be intact. These observations challenge the conventional wisdom that membrane disruption allows the browning enzyme polyphenol oxidase [catechol oxidase], which is localized in the chloroplast, to react with phenolics that leak from the vacuole.