Relationship between soil populations of Aspergillus flavus and aflatoxin contamination under field conditions in the Philippines.
Garcia, R. P.; Cotty, P. J.; Angle, J. S.; Barrios, H. A.;
ACIAR Technical Reports Series Year: 1996 Issue: No. 37 Pages: 54-60 Ref: 24 ref.
1996
บทคัดย่อ
Field plot studies were conducted during the wet and dry seasons of 1993 in Ilagan, Isabela, Philippines, to determine the relationship between populations of A. flavus in soil and the extent of infection and aflatoxin contamination of preharvest maize. Treatments consisted of 2 types of inoculation and different levels of inocula. Initial sampling of soil showed great variability in Aspergillus propagule counts within the field. Highest infection and contamination were observed in the treatment which was basally inoculated with the highest level of inoculum, indicating a direct relationship between soil population and extent of infection and aflatoxin contamination occurring in preharvest maize. The uninoculated control also became infected and contaminated, suggesting that there is sufficient indigenous inoculum level in the soil to cause infection. The extent of infection and aflatoxin contamination were generally lower during the wet than the dry season trial. Results from the vegetative com
patibility groupings analyses revealed the majority of the Aspergillus isolates from the infected grains came from the strain used as inoculum. The marked strain was transmitted into the developing maize causing infection and aflatoxin contamination.