Survey and control of the occurrence of mycotoxins from postharvest vegetables in Korea. I. Mycotoxins produced by Alternaria and Penicillium isolates from spice vegetables (onions, garlic and peppers).
Yu SeungHun, Oh SohYoung, Lee HyangBurm, Kim ByungRyun, Chung MinIll and Paik SuBong
Korean Journal of Plant Pathology Year: 1997 Vol: 13 Issue: 5 Pages: 323-330 Ref: 38 ref.
1997
บทคัดย่อ
A total of 28, 21, and 16 isolates of fungi were isolated from postharvest decayed onions, garlic and Capsicum, respectively. The predominant genus of fungi from onions and garlic was Penicillium, whereas that from Capsicum was Alternaria. The Penicillium species isolated from onions and garlic were P. aurantiogriseum, P. expansum, P. hirsutum, P. italicum and the main species of Alternaria from Capsicum was A. alternata. The production of mycotoxins in cultures of A. alternata from rice was analysed. Alternaria cultures were extracted with methanol and purified by TLC and HPLC. Among 15 isolates of A. alternata from onions, garlic and Capsicum, 14 isolates produced alternariol (AOH) and alternariol monomethyl ether (AME), 10 isolates produced altenuene (ALT) and altertoxin-1 (ATX-1), and 8 isolates produced tenuazonic acid (TA). Eighteen isolates of Penicillium from onions and garlic were tested for in vitro production of major mycotoxins. Each isolate was grown on yeast extract sucrose (YES) m
edium. The cultures were extracted with chloroform and purified by TLC and HPLC. Of the 18 isolates of Penicillium, 1 isolate of P. aurantiogriseum produced patulin and penicillic acid, 2 isolates of P. expansum produced citrinin, 2 isolates of Penicillium produced citrinin and patulin, 1 isolate of an unidentified species of Penicillium produced patulin, 1 produced citrinin and penicillic acid and another produced penicillic acid.