beta -1,3-Glucanase activity of two saprophytic yeasts and possible mode of action as biocontrol agents against postharvest diseases.
Castoria, R.; Curtis, F. de; Lima, G.; Cicco, V. de;
Postharvest Biology and Technology Year: 1997 Vol: 12 Issue: 3 Pages: 293-300 Ref: 29 ref.
1997
บทคัดย่อ
The yeasts Rhodotorula glutinis (isolate LS-11) and Cryptococcus laurentii (isolate LS-28), which show different levels of antagonistic activity against a range of postharvest pathogens, were examined for their possible mode(s) of action in order to highlight the reasons for the higher activity of isolate LS-28. Competition for nutrients appeared to play a role in the activity of both yeasts, especially in the case of isolate LS-11. Direct interaction with pathogen hyphae was shown only by cells of this same isolate, whereas no interaction with fungal hyphae was observed for the more active antagonist LS-28. The latter isolate was able to produce significantly higher levels of extracellular beta -1,3-glucanase activity in vitro than LS-11 when grown in the presence of hyphal cell walls of Penicillium expansum and Botrytis cinerea as sole carbon sources. Under the experimental conditions tested, antibiosis did not appear to be involved in the activity of either antagonists.