Nitrogenous indicators of postharvest ripening and senescence in apple fruit (Malus domestica Borkh. cv. Granny Smith).
Magne, C.; Bonenfant-Magne, M.; Audran, J. C.;
International Journal of Plant Sciences Year: 1997 Vol: 158 Issue: 6 Pages: 811-817 Ref: 37 ref.
1997
บทคัดย่อ
Effects of time and storage temperature on physiological (ethylene production) and biochemical (amino acid levels, polyamine balance, protease activity) parameters were investigated in apple cv. Granny Smith tissues during postharvest ripening and senescence. The fruit shelf life was about 90, 150 and 200 days when stored at room temperature (RT), 10 deg or 2 deg C, respectively. The climacteric peak in ethylene production was attained after 30 days at RT and 70 days at 10 deg , while apples kept at 2 deg did not show any sign of ripening. Fruit ripening was accompanied by isoleucine accumulation in peel tissue and the appearance of a nonprotein amino acid, pipecolic acid. The time relationship between these patterns in amino acids and that of ethylene production is discussed with respect to the ripening process and aspartate metabolism. In addition, the postclimacteric (senescence) period was characterized by a spermidine:putrescine ratio of 1 and, surprisingly, a steady decrease in proteolytic
activity in the pulp tissues of the fruits. The isoleucine level, the presence of pipecolic acid, and polyamine balance are proposed as metabolic indicators allowing the prediction of apple fruit stage upon storage, and consequently the shelf life.