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Asparagine synthetase gene expression increases as sucrose declines in broccoli after harvest.

Downs, C. G.; Somerfield, S. D.;

New Zealand Journal of Crop and Horticultural Science Year: 1997 Vol: 25 Issue: 2 Pages: 191-195 Ref: 19 ref.

1997

บทคัดย่อ

Asparagine synthetase gene expression increases as sucrose declines in broccoli after harvest.

Factors regulating the rapid postharvest senescence of broccoli (cv. Shogun) floral tissues were investigated. It was shown that within 6 h of harvest the sucrose concentration in florets declined by approx equal to 50%, and between 24 and 72 h asparagine levels increased more than 7-fold. This increase in asparagine paralleled an earlier increase in asparagine synthetase (AS) [aspartate-ammonia ligase] gene expression in florets. Northern analyses show that AS transcript abundance increased from 2 to 24 h after harvest, and then declined. AS transcript abundance also increased in harvested leaves as they turned yellow, although to a level lower than that seen in florets. In other plant systems, including asparagus, increases in AS gene expression occur as a result of a decline in sucrose status. The considerable similarities between broccoli and asparagus postharvest physiology are noted and the results obtained here for broccoli AS are discussed in terms of possible regulation by sucrose statu

s.