บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Isolation of low oxygen-regulated genes in tomato fruit by applying RNA differential display and yeast complementation approaches.

Aggelis, A.; Makris, A.; Christodoulidou, A.; Carrillo Lopez, A.; Kanellis, A. K.;

Postharvest Horticulture Series - Department of Pomology, University of California Year: 1997 Issue: No. 18 Pages: 149-158 Ref: 23 ref.

1997

บทคัดย่อ

Isolation of low oxygen-regulated genes in tomato fruit by applying RNA differential display and yeast complementation approaches.

The RNA differential display method was applied to isolate low-O2-regulated genes in tomato (cv. Ailsa Craig) fruits held in 3% O2 for various periods. Several clones were isolated and RNA blot analysis revealed differential expression between tomato fruits held in low O2 and air. Sequence analysis of the isolated gene fragments suggested that some of these clones are novel. Furthermore, in order to gain a better insight into the regulation of respiratory metabolism during hypoxia, the well-characterized yeast (Saccharomyces cerevisiae) anaerobic adaptation system was used to isolate putative aerobic/anaerobic plant genes. The hap locus was identified as the main regulatory complex of that response. A gene from Arabidopsis thaliana that shows 60% identity with the yeast hap5 gene and can compliment hap5-deficient yeast strains was isolated. Using a fragment of this cDNA as a heterologous probe, its expression in tomato plants was investigated. Preliminary analysis revealed that this gene is equa

lly expressed in seeds, flowers, shoots and roots and is not expressed in leaves. An increase in its expression was noticed in ripening tomato fruits.