บทคัดย่องานวิจัย

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In vitro activity of sorghum seed antifungal proteins against grain mold pathogens.

Seetharaman, K.; Whitehead, E.; Keller, N. P.; Waniska, R. D.; Rooney, L. W.;

Journal of Agricultural and Food Chemistry Year: 1997 Vol: 45 Issue: 9 Pages: 3666-3671 Ref: 24 ref.

1997

บทคัดย่อ

In vitro activity of sorghum seed antifungal proteins against grain mold pathogens.

Several sorghum (cv. Malisor 84-7) seed antifungal proteins (AFP), including sormatin, chitinase, glucanase and ribosome-inhibiting protein (RIP), were extracted, partially purified using sequential ammonium sulfate precipitation, and eluted from a CM-Sephadex column using a linear salt gradient. Eluted fractions were tested for bioactivity against Fusarium moniliforme [Gibberella fujikuroi], Curvularia lunata [Cochliobolus lunatus] and Aspergillus flavus using hyphal rupture, hyphal extension and spore germination methods. A fraction containing several AFPs was most inhibitory against G. fujikuroi, C. lunatus and A. flavus. Spore germination of all 3 species was markedly inhibited by 360 ppm of AFPs. The inhibitory effects were not observed when the protein fraction was boiled, suggesting the involvement of undenatured proteins. Hyphal elongation inhibition was the least sensitive assay to detect the inhibitory effects of AFPs on these fungi. G. fujikuroi exhibited hyphal rupture at the growing

 tip at protein levels as low as 70 ppm. C. lunatus required higher protein levels (70-360 ppm) and ruptured only at hyphal tips. A. flavus did not exhibit hyphal disruption when treated with AFPs.