Glycolytic respiratory intermediates in asparagus tips under low oxygen/high carbon dioxide atmospheres.
Silva, S. M.; Everard, J. D.; Herner, R. C.; Beaudry, R. M.;
Postharvest Horticulture Series - Department of Pomology, University of California Year: 1997 Issue: No. 18 Pages: 13-19 Ref: 9 ref.
1997
บทคัดย่อ
Asparagus spear tips were stored at 0 deg C with O2 partial pressures of approximately 0.6 or 16 kPa in combination with CO2 partial pressures of 0, 10 or 20 kPa using modified atmosphere packaging. Sugars and glycolytic intermediates were examined in spear tips at harvest and once steady-state atmospheres were reached (approximately 6 days). Analyses included sucrose, glucose, fructose, glucose-6-phosphate (G6P), fructose-6-phosphate (F6P), fructose-1,6-bisphosphate (F1,6P2), pyruvate (PYR) and phosphoenolpyruvate (PEP) content. With the exception of PYR, which remained at the initial levels, 0.6 kPa O2 caused a marked reduction in sugars and glycolytic intermediates, a trend that appeared to be independent of CO2 partial pressures. After 6 days at 16 kPa O2, G6P and F6P increased 120% to 95% relative to initial levels. The effects of CO2 were variable, but 16 kPa O2 combined with 20 kPa CO2 caused a 50% reduction in G6P relative to the content at harvest and a 3-fold decrease when compared wit
h spears placed at 16 kPa O2 in the absence of CO2. Low O2 apparently enhanced the interconversion of PEP to PYR and F6P to F1,6P2 relative to high O2. Higher CO2 enhanced the interconversion of PEP to PYR, but only at low O2. CO2 had little or no effect on the interconversion of F6P to F1,6P2.