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Changes in pomegranate anthocyanins, phenylalanine ammonia lyase and glucosyltransferase in response to carbon dioxide treatments.

Gil, M. I.; Holcroft, D. M.; Kader, A. A.;

Postharvest Horticulture Series - Department of Pomology, University of California Year: 1997 Issue: No. 17 Pages: 245-249 Ref: 6 ref.

1997

บทคัดย่อ

Changes in pomegranate anthocyanins, phenylalanine ammonia lyase and glucosyltransferase in response to carbon dioxide treatments.

The influence of CO2 on changes in aril pigmentation in pomegranate and on activity of phenylalanine ammonia lyase (PAL; EC 4.3.1.5) and UDP-glucose:flavonoid 3-O-glucosyltransferase (GT; EC 2.4.1.91) was studied in California-grown pomegranates (cv. Wonderful). Fruits were placed in jars and ventilated with air or air enriched with 10% CO2 or 20% CO2 at 10 deg C and were analysed initially, and after 1, 2, 4 and 6 weeks. Six anthocyanins (delphinidin 3-glucoside and 3,5-diglucoside, cyanidin 3-glucoside and 3,5-diglucoside, and pelargonidin 3-glucoside and 3,5-diglucoside) were identified and quantified by HPLC. CO2 treatments inhibited the growth of decay organisms compared with air-stored fruits. CO2 had no effect on pH, titratable acidity and soluble solids content of pomegranate juice. However, the total anthocyanin concentration of the fruits increased during storage in air, and to a lesser extent in air + 10% CO2, but not in air + 20% CO2. Monoglucoside derivatives from fruits held in air

 and air + 10% CO2 increased during storage while the diglucosides showed no changes. PAL activity, expressed as pkat/mg protein, increased with time at 10 deg for fruits stored in air and air + 10% CO2, reaching a maximum at 4 weeks. However, PAL activity of fruits stored in air + 20% CO2 decreased during storage. The activity of GT did not differ among treatments and storage durations.