บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Subcellular location of polyphenol oxidase in apples.

Murata, M., Tsurutani, M., Hagiwara, S. and Homma, S.

Bioscience, Biotechnology and Biochemistry Year: 1997 Vol: 61 Issue: 9 Pages: 1495-1499 Ref: 31 ref.

1997

บทคัดย่อ

Subcellular location of polyphenol oxidase in apples.

The location of polyphenol oxidase (PPO [catechol oxidase]) in cells of apple (cv. Fuji) fruits was examined by immunohistochemistry and subcellular fractionation. In mature fruits, where vacuoles occupy most of the cells, PPO was detected immunochemically near the cell walls with use of anti-apple PPO antibodies. In cells of immature fruits and in tissue culture, PPO was detected in organelles other than the vacuoles, probably in plastids. The plastid fraction was purified by density gradient ultracentrifugation, and the activities of PPO and plastid marker enzymes were highest in the plastids. Most apple PPO was in plastids, as are other plant PPOs. Some of the protein was solubilized and proteolysed during ripening and storage.