บทคัดย่องานวิจัย

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Carbohydrate status and transplant quality of micropropagated broccoli plantlets stored under different light environments.

Kubota, C.; Rajapakse, N. C.; Young, R. E.;

Postharvest Biology and Technology Year: 1997 Vol: 12 Issue: 2 Pages: 165-173 Ref: 26 ref.

1997

บทคัดย่อ

Carbohydrate status and transplant quality of micropropagated broccoli plantlets stored under different light environments.

Broccoli (cv. Green Duke) plantlets, which were ready for transplanting after photoautotrophic (sugar-free) culture, were stored for 6 weeks at 5 deg C under white, red or blue light or under darkness. Storage light intensity was 2 micro mol m-2 s-1 photosynthetic photon flux (PPF). Regardless of light quality, shoot dry weight remained unchanged when light was present during storage. Light quality affected transplant quality of the plantlets but did not affect CO2 exchange rate during storage. Stem elongation was observed for plantlets stored in red or blue light. Chlorophyll degradation was more pronounced in red or blue light than in white light or in darkness. The levels of soluble sugars (sucrose, glucose and fructose) and starch were reduced significantly in dark-stored plantlets, while they were maintained at high levels in light-stored plantlets. Red and blue light enhanced hexose (glucose and fructose) accumulation in stems during storage, which may have caused stem elongation. All plan

tlets stored in darkness died early during the following acclimatization stage. The plantlets stored in light. however, were acclimatized successfully. Regardless of light quality, illumination during storage maintained dry weight, carbohydrate levels and, therefore, regrowth potential of micropropagated plantlets during storage.