ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Soil inactivation of the Bacillus thuringiensis subsp. kurstaki cryIIA insecticidal protein within transgenic cotton tissue: laboratory microcosm and field studies.

Sims, S. R.; Ream, J. E.;

Journal of Agricultural and Food Chemistry Year: 1997 Vol: 45 Issue: 4 Pages: 1502-1505 Ref: 24 ref.

1997

บทคัดย่อ

The environmental fate of Bacillus thuringiensis subsp. kurstaki CryIIA insecticidal protein expressed within transgenic cotton plant tissue (Bt cotton) was evaluated by determining reduction in the biological activity of the protein incubated in soil for 120 days. Studies were conducted in a laboratory microcosm and under field conditions during the autumn and winter of 1995-96 in St. Louis, Missouri. An insect bioassay, based on growth inhibition of larval Heliothis virescens, was used to estimate DT50s (50% dissipation time = half-life of bioactivity) of the CryIIA protein. DT50s were 15.5 and 31.7 days for the laboratory and field, respectively. The levels of initial CryIIA protein bioactivity remaining after 40 days of incubation were similar for the laboratory and field samples. In both environments, <25% of the initial bioactivity remained after 120 days. The results indicated that the biological activity of CryIIA protein, as a component of postharvest Bt cotton plants, readily dissipate

s when cultivated into soil and suggests that laboratory soil microcosms can be useful for estimating the rate at which dissipation occurs in the field.