บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Pectin lyase and polygalacturonase isoenzyme production by Botrytis cinerea during the early stages of infection on different host plants.

Chilosi, G.; Magro, P.;

Journal of Plant Pathology Year: 1997 Vol: 78 Issue: 1 Pages: 61-69 Ref: 37 ref.

1997

บทคัดย่อ

Pectin lyase and polygalacturonase isoenzyme production by Botrytis cinerea during the early stages of infection on different host plants.

Etiolated soyabean hypocotyls, marrows and apple fruits were inoculated with 3 types of inoculum of B. cinerea (ungerminated conidia, germinated conidia and mycelium). All inoculum types caused water-soaked lesions in the plant tissues except for ungerminated conidia which gave rise to necrotic areas in soyabean seedlings. Infected tissues were sampled at various intervals after inoculation and assayed for pectin lyase (PNL) and polygalacturonase (PG) activity. After isoelectric focusing the patterns of their isoenzymes were defined. A host and inoculum age dependent PNL and PG isoenzyme sequence was observed and no PNL activity was detected in infected apple tissues. Pectolytic activity was not found in the necrotic areas induced in soyabean by ungerminated conidia, where glyceollin I, the principal soyabean phytoalexin, was recorded. Constitutive PNL and PG isoenzymes were detected in extracted conidia. The role of pectolytic enzymes, particularly PNL, from B. cinerea, detected in compatible a

nd incompatible interactions during the early stages of infection, is discussed.