บทคัดย่องานวิจัย

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Antisense apple ACC-oxidase RNA reduces ethylene production in transgenic tomato fruit.

Bolitho, K. M.; Lay-Yee, M.; Knighton, M. L.; Ross, G. S.;

Plant Science (Limerick) Year: 1997 Vol: 122 Issue: 1 Pages: 91-99 Ref: 36 ref.

1997

บทคัดย่อ

Antisense apple ACC-oxidase RNA reduces ethylene production in transgenic tomato fruit.

Transgenic tomato (Lycopersicon esculentum) plants were produced which expressed antisense copies of an apple fruit ACC oxidase RNA. In the fruit of the primary transformants, ethylene production was reduced by over 95% in one of the lines assessed, and to a lesser extent in the other lines. The line showing the greatest reduction in ethylene production showed a delay in the development of colour in the transgenic fruit. Northern analysis of steady-state RNA levels using strand-specific probes indicated that fruit of the low-ethylene line had very low levels of ACC-oxidase sense RNA, and high levels of antisense RNA. In other lines, high levels of antisense RNA were not always associated with a reduction in the levels of either the sense transcript or ethylene production. The successful reduction in ethylene production confirms the usefulness of using tomato as a model system for testing specific ripening-related genes from heterologous fruit species such as apple.