Effect of the antagonist Candida sake on apple surface microflora during cold and ambient (shelf life) storage.
Teixido, N.; Usall, J.; Gutierrez, O.; Vinas, I.;
European Journal of Plant Pathology Year: 1998 Vol: 104 Issue: 4 Pages: 387-398 Ref: 35 ref.
1998
บทคัดย่อ
The effects of the application to apples of C. sake CPA-1 (3 x 106 colony forming units (cfu)/ml) on the resident microbial populations prior to harvest were investigated. The effects on the populations were also investigated during 7 months cold storage and subsequent ambient shelf-life of fruits untreated or treated with a preharvest pesticide programme. Generally, application of the antagonist had little effect on the total bacterial populations, which remained very low both prior to harvest and during cold storage. White yeasts were predominant on the apples during the experimental period, with lower populations of pink yeasts. When apples were removed after 7 months to ambient conditions, the yeast populations increased quickly, but apples treated with C. sake had significantly less white yeasts than untreated controls. The dominant filamentous fungi isolated were Cladosporium, Alternaria and Penicillium spp. The latter, which is responsible for major postharvest diseases, was seldom isolat
ed preharvest, but it became important during later cold storage and shelf life period. Populations of Cladosporium and Penicillium were significantly reduced by the C. sake treatment when removed from cold storage during the ambient shelf-life. Alternaria spp. were unaffected by the antagonist. The actual populations of C. sake applied decreased significantly immediately after application (24 h). However, they subsequently increased to a maximum after 1 month of cold storage (103 cfu/g), and populations increased again under ambient shelf-life conditions. The C. sake populations, and the resident microbial populations, were unaffected by preharvest fungicide applications.