Preliminary study of exo- beta -1,3-glucanase encoding genes in relation to the protective activity of Pichia anomala (strain K) against Botrytis cinerea on postharvest apples.
Grevesse, C.; Jijakli, M. H.; Duterme, O.; Colinet, D.; Lepoivre, P.;
Bulletin OILB/SROP Year: 1998 Vol: 21 Issue: 9 Pages: 81-89
1998
บทคัดย่อ
In a previous study, the yeast Pichia anomala strain K was found to provide a high and reliable level of protection against Botrytis cinerea on wounded apples. An exo- beta -1,3-glucanase (paexg2), active in reducing B. cinerea germinative tube growth, was purified to homogeneity from a culture filtrate of strain K. In order to find genes coding for exo- beta -1,3-glucanase in strain K, a PCR approach using degenerated primers was used. Two PCR fragments (PAEXG1a and PAEXG2a) were found to share a significant similarity at the deduced amino acid level with exo- beta -1,3-glucanases from other fungi. PAEXG1a and PAEXG2a have 90% identity in their homologous region at the amino acid level and 80% identity at the nucleotide level. Haploid populations have been produced from the diploid strain K by ascus dissection. PAEXG1a and PAEXG2a served as probes in Southern blots on genomic DNA extracted from strain K and its haploid descendants. Segregation of PAEXG1a and PAEXG2a was discussed in relation to
the in vivo protective effect and the in vitro exo- beta -1,3-glucanase activity of the yeasts.