บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Molecular investigations into mechanisms in the biological control of postharvest diseases of citrus.

Bull, C. T.; Wadsworth, M. L.; Pogge, T. D.; Le, T. T.; Wallace, S. K.; Smilanick, J. L.;

Bulletin OILB/SROP Year: 1998 Vol: 21 Issue: 9 Pages: 1-6 Ref: 14 ref.

1998

บทคัดย่อ

Molecular investigations into mechanisms in the biological control of postharvest diseases of citrus.

Pseudomonas syringae strains ESC-10 and ESC-11 produce syringomycin and control green and blue moulds of citrus caused by Penicillium digitatum and P. italicum, respectively. Although syringomycin inhibits the fungi in vitro, it is not known what role syringomycin production plays in disease control. A lacZ reporter gene system was used to determine the influence of orange and lemon rinds on production of syringomycin E in vitro. The syrB biosynthesis gene disrupted by lacZ was used to replace the wild-type allele in strains ESC-10 and ESC-11, generating syringomycin E mutants. In cultures inoculated with the syrB mutant of strain ESC-11, beta -galactosidase activity in a minimal medium (SRM) did not increase when lemon or orange flavedo tissue were added, indicating that these tissues do not induce production of syringomycin in this strain. However, in cultures inoculated with the syrB mutant of strain ESC-10, beta -galactosidase activity was higher in media containing fruit albedo tissue after

 4 days incubation. Currently the reporter-gene system is being used to determine if syringomycin E is produced by these strains in wounds on lemons or oranges.