ศูนย์นวัตกรรมเทคโนโลยีหลังการเก็บเกี่ยว

Chlorophyll fluorescence techniques to detect atmospheric stress in stored apples.

DeEll, J. R.; Prange, R. K.; Murr, D. P.;

Acta Horticulturae Year: 1998 Issue: No. 464 Pages: 127-131 Ref: 4 ref.

1998

บทคัดย่อ

Chlorophyll fluorescence was evaluated as a rapid indicator of low O2 and high CO2 stress in stored apples. McIntosh apples were harvested in September 1994 in Nova Scotia, Canada, stored in controlled atmosphere (CA, 2.5% O2 and 4.5% CO2) at 3 deg C for approx equal to 5 months and then subjected to low O2 treatments of 1%, 2%, 4% or 8% for 1, 2, 4, or 8 days at 20 deg C. Similarly, Delicious and Golden Delicious apples were harvested in October 1994, stored in CA at 0 deg C for approx equal to 6 months and the subjected to high CO2 treatments of 5%, 10% or 20% for 1, 2, 4 or 8 d at 20 deg C. Chlorophyll fluorescence was measured by an OS-500 Modulated Fluorometer, using the Fv/Fm test (Method 1 on fluorometer) and the Fast Actinic test (Method 4 on fluorometer). Fv/Fm was found to decrease in fruits stressed with either low O2 or high CO2, while T1/2 (half-time for rise in Fv) was found to increase in the stressed fruits. The effects of low O2 or high CO2 on Fv/Fm and T1/2 were observed after

1 day, and this did not change with increased exposure time. Although Delicious and Golden Delicious apple fruits differed in their relative fluorescence yields, both cultivars responded similarly to high CO2 stress. The results indicate that chlorophyll fluorescence techniques can detect low O2 or high CO2 stress in apple fruits prior to the development of associated disorders, and thus has potential as a rapid and non-destructive method to screen for atmospheric stress tolerance in apple fruits.