Induction of chitinase and beta -1,3-endoglucanase proteins by UV irradiation and wounding in grapefruit peel tissue.
Porat, R.; Lers, A.; Dori, S.; Cohen, L.; Weiss, B.; Daus, A.; Wilson, C. L.; Droby, S.;
Phytoparasitica Year: 1999 Vol: 27 Issue: 3 Pages: 233-238 Ref: 18 ref.
1999
บทคัดย่อ
UV irradiation enhanced the resistance of grapefruit (cv. Marsh Seedless) against the development of green mould decay caused by Penicillium digitatum, the main postharvest pathogen of citrus fruit, and significantly inhibited the fungus' growth at the fruit wound sites. Immunoblotting analysis using specific citrus chitinase and beta -1,3-endoglucanase antibodies, showed that UV irradiation, wounding of the fruit, or a combination of these two treatments, induced the accumulation of a 25 kD chitinase protein in the fruit's peel tissue. In contrast, UV irradiation or wounding of the fruit alone was unable to induce the accumulation of 39 and 43 kD beta -1,3-endoglucanase proteins, but the combination of the two treatments increased these protein levels. It is suggested that both chitinase and beta -1,3-endoglucanase may play a role in the UV-induced resistance of grapefruit against P. digitatum.