บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Simultaneous identification of melon postharvest decay pathogens by the random amplified polymorphic DNA analysis with polymerase chain reaction.

Chen ShangWu; Ma HuiQin; Wang Hui;

Research progress in plant protection and plant nutrition. Year: 1999 Pages: 109-114 Ref: 10 ref.

1999

บทคัดย่อ

Simultaneous identification of melon postharvest decay pathogens by the random amplified polymorphic DNA analysis with polymerase chain reaction.

A system for the reliable identification of pathogens of melon was developed using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The pathogens tested were Alternaria alternata, Fusarium semitectum [F. pallidoroseum], F. roseum and Penicillium viridicatum. Fruits from melon cultivars Queen and Karaksai were surface sterilised and inoculated with the pathogens, after which they were tested for the presence of the pathogen by RAPD-PCR. Simultaneous identification of the pathogens from their pure genomic DNA extracts or from the diseased melon fruit peel tissues was achieved. The length of the DNA fragments produced by PCR was 150-800 bp. It is concluded that the results were stable, repeatable and sensitive and it is suggested that this system could be used as a routine method to evaluate the infection of these diseases in melon fruit.