บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Expression of ACC synthase and ACC oxidase genes in different tissues of immature and mature cucumber fruits

Shiomi, S., Nakamoto, J. I., Yamamoto, M., Kubo, Y., Nakamura, R. and Inaba, A.

Journal of the Japanese Society for Horticultural Science Year: 1999 Vol: 68 Issue: 4 Pages: 830-832.

1999

บทคัดย่อ

Expression of ACC synthase and ACC oxidase genes in different tissues of immature and mature cucumber fruits.

Ethylene biosynthesis and expression of 1-aminocyclopropane-1-carboxylate (ACC) synthase (ACS) and ACC oxidase (ACO) genes were investigated in immature cucumber fruits, harvested at 10 days after anthesis (DAA), and mature fruits, harvested at 35 DAA. During storage at 25 deg C or in response to exogenous ethylene (1000 ppm), ACS activity in fruits harvested at 35 DAA remained at low levels resulting in very little ethylene production, although ACO activity was markedly enhanced by the treatment. Northern blot analysis revealed that transcripts for CS-ACS1, a cucumber ACS gene highly homologous to CMe-ACS1 which is expressed during melon fruit ripening, did not accumulate in any mature cucumber tissues. Exogenous ethylene did not induce accumulation of CS-ACS1 transcripts but suppressed CS-ACS2 mRNA accumulation in the peel and mesocarp tissues. CS-ACO1 transcripts were increased by exogenously applied ethylene, but CS-ACO2 was constitutively expressed regardless of the treatment. The results c

onfirm that cucumber belongs to the group of non-climacteric fruits because CS-ACS1 expression was not induced during fruit ripening or in response to exogenous ethylene.