Genetic engineering of cantaloupe to reduce ethylene biosynthesis and control ripening.
Clendennen, S. K.; Kellogg, J. A.; Wolff, K. A.; Matsumura, W.; Peters, S.; Vanwinkle, J. E.; Copes, B.; Pieper, M.; Kramer, M. G.;
Biology and biotechnology of the plant hormone ethylene II. Proceedings of the EU-TMR-Euroconference Symposium, Thira (Santorini), Greece, 5-8 September, 1998. Year: 1999 Pages: 371-379 Ref: 16 ref.
1999
บทคัดย่อ
The T3 bacteriophage gene product S-adenosylmethionine hydrolase (SAMase) catalyses the degradation of SAM, a precursor to ethylene biosynthesis. Because both SAM and ethylene play a number of important roles in normal plant growth and development, a synthetic promoter was designed to restrict SAMase expression to the ripening fruit of cantaloupe. The ripening phenotype of cantaloupe transformed with a fruit-specific SAMase expression construct was analyzed in greenhouse and field trials. Results indicate that the genetically modified cantaloupe expressing SAMase exhibited modified postharvest characteristics, including a dramatic reduction in ethylene synthesis.