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Ethylene enhances the antifungal diene content in idioblasts from avocado mesocarp.

Prusky, D.; Leikin-Frenkel, A.; Madi, L.;

Biology and biotechnology of the plant hormone ethylene II. Proceedings of the EU-TMR-Euroconference Symposium, Thira (Santorini), Greece, 5-8 September, 1998. Year: 1999 Pages: 277-284 Ref: 16 ref.

1999

บทคัดย่อ

Ethylene enhances the antifungal diene content in idioblasts from avocado mesocarp.

Exposure of whole avocado fruits cv. Fuerte to ethylene (40 micro l/litre) for 3 h transiently enhanced the level of antifungal 1-acetoxy-2-hydroxy-4-oxo-heneicosa-12,15-diene (diene) in the pericarp without affecting disease resistance to Colletotrichum gloeosporioides [Glomerella cingulata]. Exposure of 1-2 mm slices of pericarp and mesocarp to ethylene enhanced the level of the diene in the mesocarp only. Since most of the diene in the mesocarp was compartmentalized in idioblasts, the effect of ethylene was tested on isolated idioblasts. Exposure of idioblasts to ethylene increased the level of antifungal diene 2-fold within 3 h. This effect was temperature dependent. Exposure of idioblasts for 3 h to ethylene at 35 deg C doubled the diene content compared with <50% increase after 3 h at 20 deg C. Incubation of idioblasts with [2-14C]malonyl-CoA or [1-14C]acetate in the presence of ethylene showed the incorporation of the label into a compound that co-chromatographed with the diene. NMR ident

ified the compound induced by ethylene and released from the cells as the diene. cDNA libraries were constructed from mesocarp tissue of ethylene-treated and untreated fruits. Using the castor stearoyl-acyl carrier protein (ACP) desaturase gene, a putative stearoyl (ACP) desaturase clone of avocado was detected. Transcripts of this clone were 2.5-fold higher in ethylene-treated than in untreated tissue.