Control of melon ripening by genetic engineering.
Guis, M.; Amor, M. B.; Botondi, R.; Ayub, R.; Latche, A.; Bouzayen, M.; Pech, J. C.;
Genetics and breeding for crop quality and resistance. Proceedings of the XV EUCARPIA Congress, Viterbo, Italy, September 20-25, 1998. Year: 1999 Pages: 307-312 Ref: 31 ref.
1999
บทคัดย่อ
In addition to its involvement in many important pathways leading to changes in colour, flavour, texture and aroma, ethylene also accelerates the senescence process resulting in poor storage quality of the fruits. Due to its key role in the ripening process, ethylene is an attractive target for controlling fruit ripening. Strong inhibition of ethylene synthesis has been achieved in Cantaloupe Charentais melons by downregulating the gene coding for the last enzyme of the ethylene biosynthetic pathway, ACC oxidase [1-aminocyclopropane-1-carboxylate oxidoreductase]. An engineered antisense ACC oxidase line with 99% inhibition of ethylene production exhibited a strong but reversible inhibition of fruit ripening. This paper reviews recent work on the molecular, biochemical and physiological characterization of this line both as pre- and postharvest levels.