Postharvest changes on cell wall and peroxidases of white asparagus (Asparagus officinalis L.).
Rodriguez, R.; Guillen, R.; Heredia, A.; Fernandez-Bolanos, J.; Jimenez, A.;
Acta Horticulturae Year: 1999 Issue: No. 479 Pages: 477-482 Ref: 7 ref.
1999
บทคัดย่อ
Changes in the cell wall polysaccharides and in the isoenzymatic distribution of peroxidases during storage of A. officinalis spears was studied. The cell wall was isolated, and its polysaccharides fractionated according with their solubility in water, imidazole/HCl buffer, sodium carbonate and KOH of different concentrations. The cellulose fraction of fresh A. officinalis spears represented approximately 50% of the cell wall. Pectic polysaccharides were rich in galactose and arabinose, and the main hemicellulosic polysaccharides were xylans and xyloglucans. During cold storage, there was a decrease in the extractability of polysaccharides, especially of those soluble in water, imidazole/HCl buffer and sodium carbonate. The isoenzymatic profiles of soluble and ionically bound peroxidases of fresh A. officinalis spears were very different; the unbound fraction was the predominant isoenzyme in the soluble fraction. However, during storage, the unbound fraction was the predominant isoenzyme in both
the soluble and ionically bound peroxidases. There were no obvious relationships between enzyme activity and texture.