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Senescence-related serine protease in parsley.

Jiang WeiBo, Lers, A., Lomaniec, E. and Aharoni, N.

Phytochemistry Year: 1999 Vol: 50 Issue: 3 Pages: 377-382 Ref: 22 ref.

1999

บทคัดย่อ

Senescence-related serine protease in parsley.

 

During leaf senescence protein degradation is enhanced. In order to obtain information on the enzymes involved in this process, a study was initiated to identify and characterize proteases whose activity is elevated in senescing Petroselinum crispum leaves. A 70-kDa serine protease (EC 3.4.21) was identified by an activity gel assay. Protease activity, which was low in young leaves, increased in parallel with advancing senescence and the reduction in leaf protein content. A high correlation between the progress of senescence and the increase in the activity of the 70 kDa serine protease was demonstrated. Treatments with CO2 or gibberellic acid, which retard senescence, reduced the activity of the protease, whereas acceleration of senescence with ethylene enhanced it.