บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Characters of postharvest physiology of antisense ACS transgenic tomato fruits.

Luo YunBo; Hao SiPing; Sheng JiPing;

Journal of China Agricultural University Year: 2000 Vol: 5 Issue: 3 Pages: 13-17 Ref: 13 ref.

2000

บทคัดย่อ

Characters of postharvest physiology of antisense ACS transgenic tomato fruits.

Postharvest physiology was studied in tomato transgenic for antisense 1-aminocyclopropane-1-carboxylate synthase (ACS). Ethylene production from either transgenic fruits or leaf disks was significantly lower than in the non-transformed control. Chlorophyll degradation and lycopene biosynthesis was inhibited in transgenic fruits. After 63 days under normal room conditions, transgenic fruits eventually developed an orange colour but never turned red. During ripening, the levels of sugar and titratable acids and the ratio of sugar to titratable acids was similar in transgenic and control fruits, but levels of ascorbic acid were lower in transgenic fruits. The firmness of transgenic fruits declined more slowly. After one month of storage, levels of sugar and titratable acids and their ratio declined greatly in transgenic fruits. In addition, polygalacturonase (PG) activity of transgenic fruits was reduced significantly at the early stages of ripening, and the maximum level of PG activity in transgen

ic fruits appeared 45 days after the breaker stage, but it was just half as much as in the control. Furthermore, transgenic tomato fruits treated with ethylene failed to show any increase of PG activity.