Control of postharvest decay of apple fruit by Aureobasidium pullulans and induction of defense responses.
Ippolito, A.; El-Ghaouth, A.; Wilson, C. L.; Wisniewski, M.;
Postharvest Biology and Technology Year: 2000 Vol: 19 Issue: 3 Pages: 265-272 Ref: 33 ref.
2000
บทคัดย่อ
The biocontrol activity of A. pullulans on decay of apple fruits (cv. Red Delicious) caused by Botrytis cinerea and Penicillium expansum, and its ability to induce biochemical defence responses in apple tissue, were investigated. In apple wounds, A. pullulans multiplied rapidly and controlled decay caused by either B. cinerea or P. expansum. At the end of 14 days' storage at 24 deg C and 95% RH, decay incidence in fruits treated with A. pullulans and then inoculated with B. cinerea or P. expansum was 11% and 33%, respectively, while 100% of the water-treated control fruits were diseased. In addition to controlling decay, A. pullulans caused a transient increase in beta -1,3-glucanase, chitinase and peroxidase activities starting 24 h after treatment and reaching maximum levels 48 and 96 h after treatment. An increase in beta -1,3-glucanase, chitinase and peroxidase activity was also triggered by wounding, although the level of increase was markedly lower than that detected in treated fruits. It
is suggested that the ability of A. pullulans to increase activities of the 3 enzymes, in addition to its known capacity to out-compete pathogens for nutrients and space, may be the basis of its biocontrol activity.