บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Evidence that α-farnesene biosynthesis during fruit ripening is mediated by ethylene regulated gene expression in apples

Ju ZhiGuo and Curry, E. A.

Postharvest Biology and Technology Year: 2000 Vol: 19 Issue: 1 Pages: 9-16.

2000

บทคัดย่อ

Evidence that alpha -farnesene biosynthesis during fruit ripening is mediated by ethylene regulated gene expression in apples.

The effect of ethylene regulation on alpha -farnesene biosynthesis in preclimateric Delicious and Granny Smith apples was studied using an ethylene inducer and inhibitor, alpha -farnesene biosynthesis precursors, and protein transcription and translation inhibitors. alpha -Farnesene was not detectable when internal ethylene concentrations were less than 1 micro l/litre. Correlations between internal ethylene and alpha -farnesene production fit the exponential growth equation and were significant in Delicious (y=e0.17; r2=0.68) and Granny Smith (y=e0.18; r2=0.83). When applied at harvest, aminoethoxyvinylglycine (AVG) at 200 mg/litre inhibited both internal ethylene accumulation and alpha -farnesene production, whereas ethephon at 200 mg/litre accelerated both. Adding ethephon to AVG-treated fruits after 18 days at 20 deg C induced internal ethylene accumulation and alpha -farnesene production. Ethephon induced alpha -farnesene production in discs from preclimacteric fruit peel as well as AVG-tre

ated fruit peel, but alpha -farnesene was undetectable when cycloheximide (CHI, 50 mM), actinomycin D (Act D, 50 mM), or silver nitrate (150 mg/litre) were added to the ethephon-treated discs. In preclimacteric fruit discs at harvest, with or without AVG treatment, alpha -farnesene biosynthesis was induced by 50 micro M mevalonic acid lactone (MAL) or farnesyl pyrophosphate (FPP), but not by hydroxymethylglutaric acid (HMG). Adding CHI or Act D to these discs did not affect the induction of alpha -farnesene by MAL or FPP.