Quantification of emboli by visualization of air filled xylem vessels.
Nijsse, J.; Meeteren, U. van; Keijzer, C. J.;
Acta Horticulturae Year: 2001 Issue: No. 543 Pages: 245-248 Ref: 6 ref.
2000
บทคัดย่อ
Segments of 30 cm length were cut from chrysanthemum (Dendranthema grandiflorum [D. morifolium] cv. Cassa) stems under water. Stems air-dried under vase life conditions (20 deg C, 60% RH) before freezing in liquid nitrogen to immobilize the water (and allow visualization of air emboli) were compared with non-desiccated control stems to determine if air enters the xylem vessels with moderate desiccation (5% w/w), and whether the entrance of air is restricted to the cut vessels. The vessel length distribution of cut stems was characterized using the latex particle method and compared to the distribution of air embolisms in a moderately desiccated stem using cryo-scanning electron microscopy. After moderate desiccation, all cut vessels were completely filled with air while the intact vessels were not.