Biological control of blue mould on apple by a strain of Candida sake under several controlled atmosphere conditions.
Usall, J.; Teixido, N.; Fons, E.; Vinas, I.;
International Journal of Food Microbiology Year: 2000 Vol: 58 Issue: 1/2 Pages: 83-92 Ref: 26 ref.
2000
บทคัดย่อ
The biocontrol potential of the yeast Candida sake (CPA-1) against Penicillium expansum decay of apples under several controlled atmosphere conditions was investigated. In a laboratory trial under different commercial cold storage conditions, increasing concentrations of C. sake improved decay control. A maximum reduction of decay was achieved at 3% O2-3% CO2 atmosphere. It amounted to a 97% lesion reduction after treatment with a suspension containing 2.4x106 CFU/ml of C. sake. In a semi-commercial trial at 1 deg C with wounded fruits, the reduction in decay diameter caused by C. sake exceeded 80% after 60 days at 21% O2 and 60% after 120 days of storage under controlled atmosphere conditions. For the seven controlled atmosphere conditions studied, a significant influence by C. sake on P. expansum decay was observed, and the lesion size was reduced more than 70% by C. sake at 107 CFU/ml. The populations of C. sake on the apple surface followed the same pattern under all controlled atmosphere co
nditions studied. They decreased 4- to 10-fold during the first 2 weeks, followed by an increase to the initial level after 45 days, and thereafter the count remained constant for the period of 90 days examined. This indicated the capacity of C. sake to colonize the surface of apples under various storage conditions. The ability to colonize was even higher in apple wounds.