บทคัดย่องานวิจัย

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Variability of three isolates of Botrytis cinerea affects the inhibitory effects of calcium on this fungus.

Chardonnet, C. O., Sams, C. E., Trigiano, R. N. and Conway, W. S.

Phytopathology Year: 2000 Vol: 90 Issue: 7 Pages: 769-774 Ref: 46 ref.

2000

บทคัดย่อ

Variability of three isolates of Botrytis cinerea affects the inhibitory effects of calcium on this fungus.

The objectives of this work were to study the variability and to compare the inhibitory effects of Ca on three isolates of B. cinerea from decayed apple (B) and grape (C and C77:4). Among these isolates, B had the least radial growth but had a sporulation rate 40% higher than that of both C77:4 and C. In situ, isolate C incited the largest decay area in the fruit of two of four apple cultivars examined and had the highest polygalacturonase activity in vitro. Maximum mycelial growth was reached with CaCl2 at 1 g litre-1 for isolates B and C77:4 and at 4 g litre-1 for isolate C. Calcium (CaCl2) inhibited polygalacturonase activity at 1 g litre-1 for C and C77:4 and at 16 g litre-1 for B. Calcium infiltration reduced decay caused by all three isolates by three to five times. Mycelial DNA analysis showed that 42% of the character loci scored were polymorphic and the greatest similarities were found between B and C77:4. These results support the evidence that the biological and statistical variabilit

y in research can be affected by the B. cinerea isolate selected. Despite this variation, Ca treatment of apples reduced decay caused by all three Botrytis cinerea isolates.