บทคัดย่องานวิจัย

Endogenous Aspergillus spp. contamination of postharvest corn in Parana State, Brazil.

Farias, A. X. de; Robbs, C. F.; Bittencourt, A. M.; Andersen, P. M.; Correa, T. B. S.;

Pesquisa Agropecuaria Brasileira Year: 2000 Vol: 35 Issue: 3 Pages: 617-621 Ref: 28 ref.

2000

บทคัดย่อ

Endogenous Aspergillus spp. contamination of postharvest corn in Parana State, Brazil.

Sixty post-harvest samples of maize kernels from three regions of Parana State, Brazil, were evaluated for endogenous fungal contamination and the toxigenic potential of Aspergillus spp. Forty apparently healthy kernels were selected from each sample, disinfested with NaClO and incubated at 25 plus or minus 1 deg C. Fungal species were isolated on Czapek-Dox agar plates. Aspergillus flavus, A. parasiticus, Eurotium amstelodami and E. chevalieri were identified. The toxigenic potential of Aspergillus species was analysed in coconut agar medium. Eurotium spp. were evaluated for their metabolites in peanut agar medium and in wheat grits. Kernel contamination varied from 0 to 100% and the prevalent genera detected were Aspergillus, Penicillium and Fusarium. A. flavus was the predominant species (64%), followed by E. amstelodami (19%), E. chevalieri (10%) and A. parasiticus (7%). From 109 isolates of A. flavus, 73 strains synthesized aflatoxins B1 and B2, 20 synthesized B1, seven synthesized B1 and G

1, three synthesized B1, B2 and G1 and in six strains aflatoxin production was not detected. All A. parasiticus species produced, simultaneously, B1, B2, G1 and G2. Sterigmatocystin synthesis was not detected in any form of E. amstelodami and E. chevalieri.