บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Analysis of post-harvest deterioration in tuberous roots of cassava (Manihot esculenta Grantz).

Huang JiAng

Thesis (PhD), Wageningen University. 2001. 103 p.

2001

บทคัดย่อ

Analysis of post-harvest deterioration in tuberous roots of cassava (Manihot esculenta Grantz).

This thesis deals with the analysis of postharvest physiological deterioration (PPD) in cassava tuberous roots at the physiological, biochemical and molecular level. By setting up a uniform visual system to monitor the induction and maintenance of PPD under the experimental conditions, amongst others physicochemical properties of starch were characterized. Furthermore using this system, around 6000 TDFs (transcript derived fragments) were screened via 100 primer combinations using the cDNA-AFLP technique. Seventy TDFs showing an upregulated, a down regulated or a transient expression pattern were isolated following the first 72 h of PPD. Based on the sequence information, a functional catalogue of these TDFs was established. By concentrating on enzymes possibly involved in oxidative stress, biochemical results indicated that PPD may be a peroxidase-mediated process. Using a reverse genetic approach, the putative cassava dad1 (defender against cell death gene 1) homologue was transformed into cassava FEC (friable embryogenic callus) lines. Transgenic plants were produced and characterized.