บทคัดย่องานวิจัย

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In wounded sugar beet (Beta vulgaris L.) tap-root, hexose accumulation correlates with the induction of a vacuolar invertase isoform.

Rosenkranz, H., Vogel, R., Greiner, S. and Rausch, T.

Journal of Experimental Botany. Volume 52, Number 365, 2001. Pages 2381-2385.

2001

บทคัดย่อ

In wounded sugar beet (Beta vulgaris L.) tap-root, hexose accumulation correlates with the induction of a vacuolar invertase isoform.

Wounding of sugar beet tap root causes an induction of invertase [ beta -fructofuranosidase] activity, which contributes to postharvest sucrose losses. Studies were conducted to identify the invertase isoform(s) responsible for the observed wound-induced hexose accumulation, by monitoring the expression of mRNAs and protein, and enzyme activity of cell wall and vacuolar isoforms (CWI and VI). The results demonstrated that the wound induction of the CWI-1 isoform clearly preceded the induction of the VI-1 isoform; however, the wound-induced increase in hexoses coincided with the delayed VI-1 induction. The expression of two other invertase isoforms (CWI-2 and VI-2) remained unaffected. It is concluded that, from a biotechnological point of view, attempts to reduce postharvest sucrose losses in sugar beet should target the VI-1 isoform.