Analysis of cellular fatty acids of Candida oleophila: studies with respect to its biocontrol activity against Penicillium expansum on apple.
El-Neshawy, S. M. and Norman, H.
ISHS Acta Horticulturae 553: 393-396.
2001
บทคัดย่อ
Preliminary experiments were conducted to determine the influence of fatty acids on the wound site produced by the biological control agent, C. oleophila, on the control of P. expansum rot in apple fruits. Combination treatments were conducted with the antagonist and pathogen in wound sites of apple fruits. Tissues from these sites were extracted and the lipid constituents were analysed by gas chromatography. Co-culturing experiments showed that C. oleophila inhibited the decay of apple fruits caused by P. expansum. The fatty acids C14:0, C16:0, C18:0, C18:1, C18:2 and C18:3 were detected in all antagonist-pathogen combination treatments. An increase in palmitic, stearic and oleic acids (C16:0, C18:0 and C18:1, respectively) content was correlated with the application of C. oleophila to wound sites, while a reduction of percentage peak area of these acids was observed in the presence of the pathogen.