บทคัดย่องานวิจัย

หมวดหมู่ : ฐานข้อมูลงานวิจัย

Modulation of sucrose-phosphate synthase activity by glucose-6-phosphate and inorganic phosphate during postharvest ripening in banana.

Pathre, U. V., Sarkar, A. K., and Nath, P.

Journal of Plant Biology. 2001. 28: pp. 301-330.

2001

บทคัดย่อ

Modulation of sucrose-phosphate synthase activity by glucose-6-phosphate and inorganic phosphate during postharvest ripening in banana.

Sucrose-phosphate synthase (SPS) activity was measured in unripe and ripe banana fruits under saturated substrate conditions and under substrate-limiting conditions in the presence of inorganic phosphate. Both the activities were low in unripe fruit and increased several fold upon ripening. The activation state of SPS (ratio of the activity measured at saturated substrate concentration and limiting substrate concentration) also showed dramatic increase from 0.15 to 0.8, indicating that during ripening the banana enzyme may be regulated via fine and coarse mechanisms as prevalent in leaf tissues. The potential for fine control of regulation of banana SPS by the metabolites inorganic phosphate (Pi) and glucose-6-phosphate (G6P) was investigated. SPS in unripe banana was inhibited strongly by Pi and activated by G6P, however their effect was less in ripe banana. The effect of these metabolites was more pronounced when both were present in the reaction mixture: the SPS activity in unripe banana was