Regulation and role of the alternative oxidase in chilling injury of green bell pepper (Capsicum annuum L.).
Purvis, A. C.
ISHS Acta Horticulturae 553: 289-292.
2001
บทคัดย่อ
Mature green bell peppers (Capsicum annuum) were stored at 15, 4 and 1 deg C for 6 to 8 days. Peppers exhibited fewer chilling injury (CI) symptoms (sheet pitting, seed darkening, increased respiration rates and decreased chlorophyll fluorescence ratios) after storage at 4 deg C than at 1 deg C. Mitochondria isolated from peppers stored at 1 deg C produced more superoxide than those from peppers stored at 4 deg C, but less than those from fresh peppers and peppers stored at 15 deg C. Western blot analysis of the mitochondrial proteins showed a substantial increase in alternative oxidase [oxidoreductase] (AOX) protein in mitochondria isolated from peppers stored at 4 deg C compared to those stored at either 1 deg C or 15 deg C. Results support a role for the AOX, along with antioxidants and the reactive oxygen species (ROS) scavenging enzymes in the mechanism by which plant tissues are protected from chilling-induced oxidative stress.